I am mainly working on the development of biosensors for diagnostics purpose by protein engineering approach. Recently, I am interesting on developing biosensor based on electrochemical enzyme and light emitting enzyme. We successfully created biosensors for immunosuppressant drugs, protein biomarkers related human diseases. Several biosensors have be planned to test for clinical samples. We believe that we can use these biosensor to build point-of-care device or biochemical diagnostics kits in clinic examination.