Personal details

Name
Dr Pradeep Deo
Position(s)
Research Associate
Science and Engineering Faculty,
School of Biology & Environmental Science
Discipline *
Agricultural Biotechnology, Crop and Pasture Production
Phone
+61 7 3138 7795
Email
Location
View location details (QUT staff and student access only)
Identifiers and profiles
ORCID iD
Qualifications

Ph.D (University of the Pacific)

* Field of Research code, Australian and New Zealand Standard Research Classification (ANZSRC), 2008

Biography

Dr. Pradeep Deo holds a PhD from The University of the South Pacific (2008) in plant tissue culture, propagation, somatic embryogenesis and transformation methodologies.  Dr. Deo has worked on
projects funded by the Bill and Melinda Gates foundation (Banana Biofortification project), Farmacule BioIndustries and the ARC. During this period Dr. Deo has developed a number of novel approaches to increasing plant biomass based on micropropagation and hydroponic techniques and has acquired a
thorough understanding of the transformation and regeneration processes for a suite of plant species. Currently he is working on QUT BlueBox POC  project involving sugarcane  transformation and Horticultural Australia Limited  funded  project on cisgenesis in potatoes.

This information has been contributed by Dr Pradeep Deo.

Experience

2011- Current  Research Associate – Farmacule Bioindustries Pty Ltd Centre for Tropical Crops and Biocommodities School of Life Sciences QueenslandUniversity of Technology,Brisbane,Australia     

Skills Developed/Responsibilities

  • Tissue culture and Agrobacterium mediated transformation of Nicotiana benthamiana for high value recombinant protein
  • Developing micropropagation system in transgenic Nicotiana benthamiana for leaf biomass production for purification of high value recombinant protein
  • Developing hydroponics culture for transgenic Nicotiana benthamiana for expression and purification of high value recombinant protein

2008 – 2010    Research Associate – Bill and Melinda Gates Foundation Centre for Tropical Crops and Biocommodities School of Life Sciences QueenslandUniversity of Technology,Brisbane,Australia

Skills Developed/Responsibilities

  • General plant cell and tissue culture
  • Initiation of embryogenic cell suspension cultures from banana male flowers
  • Screening regenerable embryogenic cell lines
  • Agrobacterium mediated transformation and selection of transgenic banana
  • Molecular characterization of transgenic banana by PCR and Southern blot
  • Gene expression studies and promoter activity analysis in transgenic banana by MUG assay

2007 to 2008

  • Visiting Academic – for transformation work (18 months) Centre for Tropical Crops and Biocommodities School of Life Sciences Queensland University of Technology, Brisbane, Australia

Skills Developed/Responsibilities

  • Media preparation (plant tissue culture and Agrobacterium culture)
  • Initiation of embryogenic suspension culture and optimizing factors
  • Regeneration of somatic embryos, maturation and germination factors
  • Transformation via biolistic and Agrobacterium tumefaciens
  • Maxi preparation of plasmid DNA, Agrobacterium culture
  • Kill curve experiments, selection and regeneration of transformed taro
  • Characterization of transgenic taro using PCR and Southern blot.

2005 to 2006

  • Graduate Assistant – Biology (PhD Internship), Division of Biology (SBCES) University of the South Pacific, Suva, Fiji.

Skills Developed/Responsibilities

  • Tutor (2 hours per week) – cell molecular biology, microbiology and genetics for first year degree students
  • Tutor (1 hour per week) and lab demonstrator (4 hours per week) – genetics for second year degree students
  • Marking lab reports – genetics for second year degree students

2004

  • Temporary Full Time Tutor (six months), Division of Biology (SBCES), University of the South Pacific, Suva, Fiji

Skills Developed/Responsibilities

  • Tutoring on cell molecular biology, microbiology and genetics for first year degree students
  • Lab demonstration– in charge of lab classes, giving lab talk to first and second year degree students
  • Marking lab reports, keeping records of practicals and tests marks

2004

  • Research Assistant (six months), Division of Biology (SBCES), University of the South Pacific, Suva, Fiji

Skills Developed/Responsibilities

  • Taro Breeding – carry out hand pollination, seed collection, seed drying and storage
  • Determine seed moisture content, conduct seed germination experiments
  • Study seed storage behaviour and determine seed viability

2003

  • Graduate Student Research in Biochemistry (3 months), Institute of Applied Science, University of the South Pacific Suva, Fiji

Skills Developed/Responsibilities

  • Extract genomic DNA from marine sponges and soft corals using CTAB protocol and QIAGEN DNA extraction kits
  • Run PCR and gel electrophoresis
  • 1998 to 2001Teacher (Secondary), Ministry of Education, Fiji

Skills Developed/Responsibilities

  • Assistant teacher – prepare lessons and practical classes for chemistry, biology and basic science
  • Form teacher – mark daily attendance, check and supervise classroom and area duty, prepare individual students progress reports and discuss student´s performance with parents
  • Teacher on Duty – supervise general school duties
  • Acting head of science – monitor smooth running of science department, keep records of science inventory, hold monthly departmental meetings to discuss students and teaching progress, managing science labs
This information has been contributed by Dr Pradeep Deo.